MacConkey Agar – Selective & Differential Medium for Gram-Negative Bacteria

Brand: BKMAM
Item Code: 110701067
Packaging: 250 g / bottle, 20 bottles/carton                                                                                Application: Gram-Negative Bacterial Isolation | Enterobacteriaceae Detection | Clinical Microbiology | Food Microbiology Testing | Water & Environmental Microbiology | Research Laboratories.
Documentation Available: COA, MSDS TDS available upon request

Original price was: ৳ 4,000.00.Current price is: ৳ 3,600.00.bottle

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Description

MacConkey Agar is a selective and differential microbiological culture medium used for the isolation and differentiation of Gram-negative enteric bacteria, particularly members of the Enterobacteriaceae family.

The medium contains bile salts and crystal violet which inhibit the growth of most Gram-positive organisms, while lactose and neutral red indicator allow differentiation between lactose-fermenting and non-lactose-fermenting bacteria.

Lactose fermenters such as Escherichia coli produce pink to red colonies, whereas non-fermenters such as Salmonella and Shigella produce colorless colonies.

MacConkey Agar is widely used in clinical microbiology, food microbiology, environmental monitoring, and water quality testing laboratories.

Key Features

  • Selective medium for Gram-negative bacteria
    • Differentiates lactose fermenters and non-lactose fermenters
    • Inhibits most Gram-positive microorganisms
    • Widely used in clinical, food, and environmental microbiology
    • Supports growth of Enterobacteriaceae species
    • Reliable colony differentiation with clear color reaction

Typical Composition (Per Liter)

Component Amount
Peptone 17 g
Proteose Peptone 3 g
Lactose 10 g
Bile Salts 1.5 g
Sodium Chloride 5 g
Neutral Red 0.03 g
Crystal Violet 0.001 g
Agar 13.5 g
Final pH (25°C) 7.1 ± 0.2

Preparation Instructions

  1. Suspend approximately 50 g of MacConkey Agar powder in 1 liter of purified or distilled water.
  2. Heat with constant agitation until the medium dissolves completely.
  3. Sterilize by autoclaving at 121°C for 15 minutes.
  4. Cool to 45–50°C and mix well.
  5. Pour into sterile petri dishes and allow to solidify before use.

 

Applications

  • Isolation of Gram-negative enteric bacteria
    • Differentiation of lactose fermenting bacteria
    • Clinical microbiology laboratories
    • Food safety and quality testing
    • Water and environmental microbiology
    • Detection of Enterobacteriaceae contamination

Storage Conditions

Store the dehydrated medium in a tightly closed container at 10–30°C in a dry environment, protected from moisture and direct sunlight.

Prepared plates should be stored at 2–8°C and used within recommended laboratory timeframes.

Quality Documentation

To support laboratory procurement, regulatory compliance, and quality assurance, the following documentation is available upon request:

  • Certificate of Analysis (COA)
    • Material Safety Data Sheet (MSDS)
    • Technical Data Sheet (TDS)

These documents provide batch specifications, safety information, technical parameters, and product performance data required by laboratories, universities, and industrial research facilities.

Available Packaging

  • 250 g Laboratory Pack
    Other pack sizes may be available depending on supplier production batches.

Why Choose GreenXsupply?

GreenXsupply is a reliable supplier of laboratory consumables and microbiology culture media in Bangladesh.

Advantages of sourcing from GreenXsupply:

  • Genuine BKMAMLAB microbiology media
    • Competitive pricing for research labs and diagnostic centers
    • Ready stock for fast delivery across Bangladesh
    • Support for universities, research institutes, and biotech labs
    • Growing portfolio of laboratory reagents and microbiology products

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